Updated post with a bit more info…
The first-line gluten test I recommend is the Cyrex Array 3, which looks for the traditional gluten proteins (gliadin, 33 mer) and the coeliac transglutaminase (Ttg2), plus importantly a lot of other markers the mainstream tests miss – and which I find come up positive a lot!
In essence, we are doing a much more comprehensive coeliac and gluten related disorders test, looking for evidence of what type of gluten problem it is – is it wheat-sensitivity only, a problem with gliadin, has villi autoimmune damage already started, is it more likely non-coeliac gluten sensitivity, are other transglutaminases, namely 3 (skin) or 6 (neurological/general) affected, is gluten likely to affect moods and behaviour, is it a likely trigger for leaky gut?
That’s a lot of info from one test.
In a mainstream coeliac test, all you would find out is whether you are reacting to one specific gliadin peptdie (33 mer) and whether it has triggered an issue with transglutaminase 2, which is linked to villi damage. Hence me now asking people to go straight to Cyrex 3 or certainly challenge a negative mainstream test result if they still suspect a gluten issue, especially men who seem to have higher false negatives than women for some reason.
Anyway, happily, Cyrex 3 has just had four new markers added to it, which makes it even better. Even more happily, I have been able to reduce the cost for you by a very pleasing £45 – more markers for less money, yay 🙂
The lab explains:
New research from Dr. Aristo Vojdani, PhD, MSc, CLS, chief scientific advisor for Cyrex Laboratories, the renowned pioneer of using ELISA for food immune reactivity and autoimmunity testing shows that as much as 20 percent of the population may be reacting to these newly added antigens. Dr. Vojdani has broken ground in the development of these new critical biomarkers for screening through Cyrex Laboratories.
He has identified gliadin toxic peptide as absolutely essential in gluten reactivity testing. This peptide lies within the larger gluten protein and has proven to be exceptionally resistant to digestion. The ability to identify a reaction to gliadin toxic peptide allows patients to remove the trigger before the intestinal barrier becomes damaged and causes serious long-term health issues.
Array 3X also includes testing for microbial (non-tissue) transglutaminase in addition to transglutaminase 2, 3 and 6. Microbial transglutaminase is an enzyme produced by bacteria, which can send signals impacting brain and nervous system function. Microbial transglutaminase can be exceptionally antigenic in some patients. Patients who consume gluten substitutes like gluten-free pasta may have a reaction to the non-tissue transglutaminase contained within these foods—which may initiate autoimmune reactivity against the other transglutaminases.
“Array 3X goes beyond any gluten immunological screen physicians have had access to in the past, and is the only test panel available for measuring antibodies against gliadin toxic peptide and microbial transglutaminase. This is a very significant step forward in providing a more comprehensive look at gluten/wheat reactivity in patients,” said Jean Bellin, president of Cyrex Laboratories. “These biomarkers can provide new critical insight to physicians due to the antigens’ possible harmful effect on both the brain and nervous system, as well as the potential damage to intestines resulting in celiac disease, and non-celiac gluten sensitivity,” added Bellin.
- Non-Gluten Proteins (A and B) — May suggest involvement in the pathogenesis of Irritable Bowel Syndrome (IBS) and is often associated with triggers of allergic disorders.
- Gliadin Toxic Peptides — A strong indicator of possible Celiac disease, Crohn’s disease and other chronic inflammatory processes.
- Microbial Transglutaminase — May send signals impacting brain and nervous system function, sometimes contributing to neurological disorders. Also, when found in gluten-free substitutes, may negate the effectiveness of a gluten-free diet in some patients.
You can see all the Cyrex tests here. Do read the Overview on that page too as it explains all about gluten testing and took me a long time to write 😉