New Active #B12 and #Anaemia Tests

As you can tell, I am catching up with stuff I haven’t been able to do with my Adrenal Plan writing hat on!

Hot on the heels of the new NeuroHormone tests, I have also changed the Vitamin B12 and Anaemia tests, which I know again that some of you have been waiting for me to do!

Why have I changed them?

Well, I have been coming across quite a few people who test fine on serum B12, but who quite clearly have B12 deficiency symptoms and who go on to improve on a B12 supplement trial.

Of course, I had to look into it. And, as Providence or someone or other would have it, TDL labs was also doing the same thing and had just introduced a new Active B12 test. Basically, like the red blood cell mineral tests, this looks for the metabolic levels of B12 rather than what is just in your blood. Here is some info on that for you (bit techy, but you get the point):

What is Active B-12?
Three carrier proteins are involved in the transport of Vitamin B12 around the body – Intrinsic Factor (IF), transcobalamin (TC) and haptocorrin (HC).

When transcobalamin and haptocorrin bind Vitamin B12 the resulting complexes are known as holotranscobalamin (HoloTC) and holohaptocorrin (HoloHC).

 Holotranscobalamin represents only 10-30% of the Vitamin B12 circulating in the blood but is the ONLY form of Vitamin B12 that is taken up and used by cells of the body, hence it’s other name… ACTIVE-B12.

 Only transcobalamin transports Vitamin B12 from its site of absorption in the ileum to tissues and cells. The vitamin is then internalised as the Active-B12 (vitamin B12 bound to transcobalamin) complex via a specific receptor-mediated uptake. This process delivers Vitamin B12 into the cells of the body and provides the vitamin as an essential co-enzyme for vital cellular functions such as DNA synthesis..

The remaining 70-90% of circulating Vitamin B12 is bound to haptocorrin, the function of which is unknown.

Because up to 90% of circulating Vitamin B12 is bound tohaptocorrin and is therefore biologically unavailable for most cells, the traditional Total B12 test can give a misleading representation of the patient’s Vitamin B12status. Active-B12, the part of Vitamin B12 bound to transcobalamin (TC), is the portion that delivers Vitamin B12 to the tissues of the body.

Also, Active-B12 has a shorter circulating half-life compared to holohaptocorrin so the earliest change that occurs on entering negative vitamin B12 balance is very likely to be a decrease in Active-B12 concentration

So, a no-brainer then to change to measuring active B12. You can see the new Vitamin B12 test here for more info.

I also wanted to extend the anaemia test to include the new active B12, but also red cell folate instead, again, of the usual serum type, and a few other indices too, including ESR for inflammation since high levels in the body can lead to misleading iron results. It’s all very technical, y’know; I don’t just make this stuff up ;)

Anyway, I think the new anaemia test is much improved on the last one but I can still do the previous Genova one if you need to do comparisons, so just ask. I’ve taken it off the shop site so I don’t confuse with having two anaemia tests.

OK, see the new Active B12 test here and the new Anaemia test with active B12 and red cell folate here.

Blimey, doing those hormone, neurotransmitters, adrenal, cortisol, B12 and anaemia test changes has taken me all day and I am shattered now! Hope they help you; am rather pleased with myself :)

5 Replies to “New Active #B12 and #Anaemia Tests”

  1. Hi Micki, I had the ‘Active B12’ test and my result was very confusing because having B12 def symptoms I was not expecting my Active to be at 50-60% of total. Any test to understand what is going on?
    I presume a problem with transport of Active into cells and so building up in the blood?

    1. Don’t know Lynne. In that kind of case, I often suggests a B12 supplement trial – your body will show you if there is an issue as you will improve; we cannot always know the exact mechanisms of what is going on, and do we actually need to…?

  2. Yes Micki, I have been supplementing for 12months now. Methyl and more recent months methyl and adenosyl Think a little improved but not always easy to determine. Not a gut absorption issue as Total serum B12 has risen with supplementation and NHS relevant tests negative/show no issue
    I am presuming possibly a a TC11 transport into cells issue hence active B12 build up/or high in blood, but no one seems to be able to advise/understand. Others with own similar issue seem to be going with supplement high and hopefully with the law of averages some B12 will get into cells.

    Also there seems to be a concensus that once you are deficient (whatever that may be for the individual) that deficiency starts to cause immediate damage and that by the time symptoms are felt or you have a low serum B12 that the deficiency has taken place and been causing damage for 10yrs+. And that the damage takes a long time to repair.
    Also that any repair cannot commence below a total serum level of 1000+ ng/L or much higher. Would you agree Micki?
    Have you seen a time factor with any of your paients?

    Also that we need to be top of the range or above for adequate function and it is noted that the Japanese who have far, far lower heart disease and dementia/alzheimers have a min total serum level of 500 or there is intervention (range 500 – 1300)
    Whereas UK is (197 – 760ng/L)
    7 years ago I went to the GP with symptoms and asked for B12 blood test (never having supplemented) GP fed back – my B12 level was normal. I later found when accessed written results that my total B12 had been 191ng/L the very bottom of the UK range at that time.

  3. Doesn’t surprise me. I constantly see low normal ranges not mentioned. You know more than me about B12 I think – often the way when you suffer yourself. I have just usually done the same advise – try and get in what you can and correct folate levels, which are needed to absorb B12 well I believe. Most of the time, I have seen B12 come up so yours must have another issue going; you’re right. I would chat on the TrulyGlutenFree group where others may recognise it.

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